Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How adjacent or remote CpG affects expression?


  • Please log in to reply
5 replies to this topic

#1 epigenetics

epigenetics

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 68 posts
0
Neutral

Posted 25 March 2009 - 09:27 AM

I got one conceptual question.
If the CpG island is not overlapping with Promoter region, But if it is adjacent to the promoter, then how does it affect gene expression? By binding to some proteins?

If CpG island is remote, then does it matter?
Reference to any paper will be appreciated. I am alos looking into.
Thanks,

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 31 March 2009 - 08:32 PM

If the CpG island is not overlapping with Promoter region, it is very likely the CpG region actually harbors the promoter sequence or alternative promoter. If you lax the criteria for the CpG island a bit, probably the island will extend into the known promoter. I would say CpG island is very indicative of promoter.

#3 ston

ston

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 01 April 2009 - 08:49 AM

Methylated CpG islands can also recruit chromatin remodelling proteins. These islands probably do not have to be in the promoter region to have an effect.

#4 epigenetics

epigenetics

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 68 posts
0
Neutral

Posted 01 April 2009 - 10:00 AM

Methylated CpG islands can also recruit chromatin remodelling proteins. These islands probably do not have to be in the promoter region to have an effect.

thanks to all of you,
But i could not find out any paper where they mentioned explicitly how the CpG island which is remote from promoter is affecting.
Got one paper where they said short sequence of CG residues in promoter can act with remote CpG island. But how they did not mention.
Thanks though.

#5 methylnick

methylnick

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 245 posts
4
Neutral

Posted 02 April 2009 - 01:54 PM

H19/IGF are the prime examples of this and the binding of CTCF in a methylation dependent manner, this binding region is a CpG island and it can effect the expression of a gene remote from it.

#6 epigenetics

epigenetics

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 68 posts
0
Neutral

Posted 06 April 2009 - 06:22 AM

H19/IGF are the prime examples of this and the binding of CTCF in a methylation dependent manner, this binding region is a CpG island and it can effect the expression of a gene remote from it.


thanks Nick, it was really helpful to find out the answer of my question.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.