a doubt about PCR gel purification
Posted 25 March 2009 - 07:16 AM
I want ask you a very simple question. I´m going to use the Qiagen gel extraction kit to purify a PCR amplified product from an agarose gel with thidium bromide, and I want ask you: how do you avoid ethidium bromide contamination using this kit? Do you do "something" betwen cutting the gel and putting it into the tube?
Posted 25 March 2009 - 07:43 AM
Posted 25 March 2009 - 07:50 AM
thanks, but I mean how take ethidium bromide out from DNa, is it possible?
As far as I know the kit does that for you. Between gel and kit you dont need to do anything, and then your DNA is ready for any downstream use you need.
Posted 25 March 2009 - 08:22 AM
Career In Life Science