Posted 06 June 2001 - 09:00 PM
Posted 23 March 2002 - 07:59 AM
You can try the DIG-labelling kits from Roche or directly label your target DNA by PCR reaction with 5'-DIG labelled primer. It is easy and gives good results.
For more information see the site of Roche (Boehringer Mannheim).
I have tried it and find it very useful.
Posted 06 January 2005 - 12:36 PM
Then, it is only necessary to perform your favorite method for the binding reaction, run the gel, perform a transfer, and detect the DNA using a streptavidin system (I have had good results detection non-radioactive DNA with kits and/or reagents from both Pierce and NEB). I use non-radioactive EMSA and I can go from labeled probe and nuclear extract to photographing the results in well under 24 hours.