Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Silver sataining kits troubleshouting


  • Please log in to reply
3 replies to this topic

#1 dr_marie

dr_marie

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 24 March 2009 - 10:59 AM

i stained today a gel using silverxpress kit but the stainer run out so i used silverquest stainer but after addition of silverxpress developer nooo bands appear!! is there a solution to solve this problem like destaining and restain again !! these samples are v important and cannot be repeated

#2 neuron

neuron

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 187 posts
5
Neutral

Posted 24 March 2009 - 09:10 PM

Hi,

we don't use any kit for silver staining, we do it manually using vorum's silver staining protocol. And I've never destained the silver stained gel, but I've heard people do it with farmer's reagent which contains potassium ferricyanide and sodium thiosulfate, you can try it. I use it for destaining the gel plugs for mass spectrometry, i think it should work with the gel. If you don't want to try straight away on your precious gel, then may be you can cut a part which doesn't have any protein and then give it a try. Here is the method to make it-

http://proteomics.ar.....Gel Bands.pdf


Hope it helps:)

Attached Files



#3 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,746 posts
128
Excellent

Posted 25 March 2009 - 06:47 AM

i stained today a gel using silverxpress kit but the stainer run out so i used silverquest stainer but after addition of silverxpress developer nooo bands appear!! is there a solution to solve this problem like destaining and restain again !! these samples are v important and cannot be repeated


i would guess that the two kits use different formulations and protocols and are not compatible.

you should use one or the other for the complete protocol.

try restaining.
talent does what it can
genius does what it must
i do what i get paid to do

#4 dr_marie

dr_marie

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 25 March 2009 - 10:01 AM

thanks a lott for your advices, i destained the gel using the destaining solution in the kit and restained again manually and it worked well !




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.