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Yeast and LB?


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4 replies to this topic

#1 mastermi

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Posted 24 March 2009 - 10:08 AM

Hi there,

does yeast like S. cerivisieae grow in standard LB-Media?
I don't need them to grow perfectly, I just want to see colonys on agar plates.

Thanks.

#2 perneseblue

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Posted 24 March 2009 - 11:18 AM

Hi there,

does yeast like S. cerivisieae grow in standard LB-Media?
I don't need them to grow perfectly, I just want to see colonys on agar plates.

Thanks.


Probably yes. But the lack of added glucose, means it probably won't grow very fast.
May your PCR products be long, your protocols short and your boss on holiday

#3 molgen

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Posted 25 March 2009 - 10:00 AM

I tried it once by mistake.
It didn't grow well at all.

#4 mastermi

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Posted 26 March 2009 - 04:24 AM

Probably yes. But the lack of added glucose, means it probably won't grow very fast.


So, if I would add Glucose to LB, E. coli wouldn't care, and my chances thet yeast would grow on it are much better?

#5 Julio-Claudian

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Posted 26 March 2009 - 05:01 AM

Done it once with a mate's yeast. Did grow on LB without glucose. Flat, white, and dry-looking (think powder) colonies.. :P




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