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integrin staining /adherent cells


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#1 isa44

isa44

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Posted 24 March 2009 - 09:26 AM

I'm trying to stain integrins on cell surface of adherents cells. I'm using Accutase to detach my cells. The antibody I'm using recognize the active forme of beta1 integrin but I cant' make it work :) . I'm staining my cells after stimulation and after detachment. Any suggestions??

Many thanks
:(




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