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[Euclid]

I have a blunt ended insert I need to clone into a vector that was cut with a sticky end restriction enzyme.  Using a blunt end enzyme to cut the vector is not an option.  I cut the vector and filled in with klenow.  Do I need to treat the linear, filled in vector with CIP?  I do not think there are phosphates on the ends after fill in, because when I do this with to an insert I need to add phosphates with PNK.

Anybody have experience with this technique and able to share it?

Thanks!