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how to design PCR site-directed multiple mutagenesis

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#1 lufang



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Posted 24 March 2009 - 03:47 AM

the cloned gene is about 1kb, there are 8 codons i want to change by PCR method. but these codons were scattered in the sequence. i read several articles on overlapping or megaprimers PCR, could some people give me some ideas or experiences how to carry out this? or it's difficult to realize?

#2 phage434



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Posted 24 March 2009 - 07:01 AM

Have you considered having it synthesized with the changes? $750 will do it, and it may be worth it, given your time.
Geneart, DNA2.0, Blue Heron would all love to do this for you, perhaps for less.

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