the cloned gene is about 1kb, there are 8 codons i want to change by PCR method. but these codons were scattered in the sequence. i read several articles on overlapping or megaprimers PCR, could some people give me some ideas or experiences how to carry out this? or it's difficult to realize?
0
1 reply to this topic
#2
phage434
-
- Global Moderators
-
- 1,808 posts
Veteran
130
Excellent
Excellent
Posted 24 March 2009 - 07:01 AM
Have you considered having it synthesized with the changes? $750 will do it, and it may be worth it, given your time.
Geneart, DNA2.0, Blue Heron would all love to do this for you, perhaps for less.
Geneart, DNA2.0, Blue Heron would all love to do this for you, perhaps for less.
