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[molbio]

Hi,

I want to do quantitative methylation analysis. I think that I will use methylight for this. I have read some articles and see that one could either use primers without CpG sites and then probes that are specific for methylated or unmethylated CpG sites or primers that are specific for methylated and umethylated sequences and then a common probe.
Which is the best strategy? When do you use which method?

I appreciate any advices on the methods!