How many bp should be left at 5' and 3' flanking regions needed for miRN
Posted 23 March 2009 - 08:24 PM
How many bp should be left at 5' and 3' flanking regions to constucting a miRNA vector?
Some people left 600bp, some people left 100 or 200 bp. How many should be enogh for expressing miRNA?
Is there any article about this?
Posted 24 March 2009 - 03:31 AM
If a longer bp was remained, any problem will happen?
Posted 24 March 2009 - 03:32 AM
100-200 bp flanking the precursor miRNA should be fine.
I tried 30 and 60.....30 was more than enough!!
but maybe i was just lucky.
Posted 03 April 2009 - 08:05 PM