I am anxious to know.
How many bp should be left at 5' and 3' flanking regions to constucting a miRNA vector?
Some people left 600bp, some people left 100 or 200 bp. How many should be enogh for expressing miRNA?
Is there any article about this?
Thank?
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How many bp should be left at 5' and 3' flanking regions needed for miRN
Started by newmirnaman, Mar 23 2009 08:24 PM
4 replies to this topic
#2
pcrman
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Posted 23 March 2009 - 10:01 PM
100-200 bp flanking the precursor miRNA should be fine.
#3
newmirnaman
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Posted 24 March 2009 - 03:31 AM
Thank you pcrman.
If a longer bp was remained, any problem will happen?
Thanks.
If a longer bp was remained, any problem will happen?
Thanks.
#4
Fizban
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Posted 24 March 2009 - 03:32 AM
pcrman, on Mar 24 2009, 07:01 AM, said:
100-200 bp flanking the precursor miRNA should be fine.
I tried 30 and 60.....30 was more than enough!!
but maybe i was just lucky.
Fiz
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Posted 03 April 2009 - 08:05 PM
I have cloned and generated over 600 human lentiviral miRNA collection. There are 100bp flanking sequences on both sides of pre-miRNA and they work great.
