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How many bp should be left at 5' and 3' flanking regions needed for miRN


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#1 newmirnaman

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Posted 23 March 2009 - 08:24 PM

I am anxious to know.


How many bp should be left at 5' and 3' flanking regions to constucting a miRNA vector?


Some people left 600bp, some people left 100 or 200 bp. How many should be enogh for expressing miRNA?


Is there any article about this?

Thank?

#2 pcrman

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Posted 23 March 2009 - 10:01 PM

100-200 bp flanking the precursor miRNA should be fine.

#3 newmirnaman

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Posted 24 March 2009 - 03:31 AM

Thank you pcrman.

If a longer bp was remained, any problem will happen?
Thanks.

#4 Fizban

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Posted 24 March 2009 - 03:32 AM

100-200 bp flanking the precursor miRNA should be fine.


I tried 30 and 60.....30 was more than enough!!
but maybe i was just lucky.
Fiz

#5 Functional Screens

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Posted 03 April 2009 - 08:05 PM

I have cloned and generated over 600 human lentiviral miRNA collection. There are 100bp flanking sequences on both sides of pre-miRNA and they work great.




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