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Activity of insulin and pro insulin

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#1 miraclestrain



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Posted 23 March 2009 - 05:20 AM

Can anyone help me out with this question with the exact answer.

Insulin consists of a shorter A chain and a longer B chain. The two chains are held together with disulfide bonds. Invitro, insulin is processed from proinsulin a single polypeptide chain. The C chain is removed from proinsulin to form insulin.

A denaturation and renaturation experiment using urea and @- mercaptoethanol was carried out usin insulin. Less than 10% of the activity of insulin was recovered when the urea and 2 Mercaptoethanol were removed by dialysis. However, when the experiement was repeated with proinsulin, full activity was restored upon renaturation. Explain the above?

PLease find attached a very simple structure of proinsulin. Try to limit the answer to the diagram. But also try to explain in detail

thanks and regards

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Edited by miraclestrain, 23 March 2009 - 05:28 AM.

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