I transformed Arabidopsis with a binary construct via agrobacterium mediated in planta method. I collected the seeds and grown on Kanamycin and selected the transgeic plants (T1). I n order to make homozygous plants , I selected seeds again on Kanamycin from T1 plants. 2 lines are showing both resistant and susceptible plants, indicating presence of homozygous/heterozygous and escapes. But the remaining 2 lines, all the plants are kanamycin resistant. How could this happen? is there any possibility of getting homozygous in T2 itself?? Any help or suggestion in this regard will be highly appreciated
Edited by theen, 23 March 2009 - 03:54 AM.