I realize that most likely the vector will close up on itself upon ligation (even though I plan on using a phosphatase prior to ligation). My real question is whether there is a decent chance that I can favor the insertion of A+B into the vector, at all to be able to detect it. Has anyone had any success with something like this or is it practically impossible?
Could I try and ligate the inserts (A and B) together first and then add vector to the ligation mix?
Thanks for the soundboard.
Edited by yoda1311, 21 March 2009 - 09:46 PM.