Posted 21 March 2009 - 11:15 AM
I swirled 10X PBS stock that I used to make inoculum and saw particles that form tornado-like shape.
Looks similar to bacterial growth, but I don't think bacteria can grow in it without any nutrient. Its just salt solution.
So, I was wondering it may be just the salt precipitating out of solution upon temperature fluctuations or something.
And if it is really the salt precipitation, can I just use it like that? Re-autoclave? Filter?
I await your suggestions.
Posted 21 March 2009 - 10:39 PM
Heat sterilization sometimes cause the salts of PBS to precipitate out. Better avoid it.
Posted 22 March 2009 - 12:55 AM
I don't think that thy can in x10 because of the osmolarity.
We always filter sterilize it in any case.
Posted 26 March 2009 - 08:03 AM
Posted 26 March 2009 - 11:01 PM
Posted 27 March 2009 - 10:34 AM
Posted 27 March 2009 - 01:02 PM
Try putting your 10x stock PBS into a water bath at 68C or a hybridisation incubator set at 68C. It should go into solution
Posted 28 March 2009 - 05:27 AM
Posted 29 March 2009 - 05:05 AM
PBS is so cheap!!!
Why don't you just buy it??
Posted 31 March 2009 - 03:15 AM
After autoclave sterilization I once saw a precipitate! It seems that the DI water installation has broken down and my water contained to much carbonates or something!
At 4 degrees celsius your salts precipitate and form christals. Heat it up again and they dissapear.
Posted 31 March 2009 - 06:56 AM
if there is calcium present (from rinsing with tap water instead of deionized, or if the deionizer is exhausted) then you may be seeing calcium phosphate precipitating (or magnesium phosphate if it was heated).
genius does what it must
i do what i get paid to do