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subcellular fractionation problem


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#1 yobou

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Posted 21 March 2009 - 06:51 AM

Dear all
I have used PIERCE kit for subcellular fractionation to prepare nuclear and cytosolic extracts. Although I followed the kit instructions but the fractions were not clean as I found beta actin in both fractions. Does anyone knows a better kit for preparation of cytosolic, nuclear and mitochondrial fractions?
one more thing, can I use syringe for homogenization instaed of vortexing?
thanks

#2 Sharon Pek

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Posted 23 April 2009 - 11:32 PM

I was told that beta actin can be sometimes found in mitochondria (amazingly....)
And tubulin might be a better control instead




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