Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

siRNA transfection of RS4-11 and MV-4-11 Cells


  • Please log in to reply
1 reply to this topic

#1 Karen2

Karen2

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 20 March 2009 - 01:20 PM

Hello,
Has anyone successfully transfected either RS4-11 or MV-4-11 cells using siRNA with a efficiency of >85%? Right now I am having problems with electroporation....most of my cells die. I am following the protocols from Amaxa on these cell lines, but even they say that transfection efficiency is poor.

I would actually prefer to use some sort of cationic/lipid based transfection reagent. Has anyone tried lipofectamine 2000 or Hiperfect or something similar in these cell lines?

Also, has anyone tried using a lentiviral shRNA for gene knockdown in these cell lines?

Thanks
Karen

#2 scolix

scolix

    a student

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 314 posts
4
Neutral

Posted 21 March 2009 - 10:24 PM

check literature. you could also check with the technical support for any transfection reagent.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.