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Co-transfection with PKJ-neo (evans et al), stable transfection


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#1 drevilette_R

drevilette_R

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Posted 20 March 2009 - 02:11 AM

Hey there!
has anyone done or heard of the co transfection with PKJ-neo for a stable transfection?? this method was first described by Evans et al in 1993.
i keep finding articles that cite this paper and use the method itself, but not the original paper describing the method or why to use it , or describing this PKJ-neo plasmid! i also cant find a map of it anywhere! :)
do i have to use it if i already have a plasmid with a neomycin/ g418 resistance in it , cloned with my gene of interest.
this is getting really confusing!
thanks for the help in advance!




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