Difference between Nonidet-P40 and Tween20 and TritonX100
Posted 19 March 2009 - 11:19 PM
Posted 20 March 2009 - 07:27 AM
Tween-20 is a polyoxyethylene surfactant.
Triton X-100 is a polyethylene oxide surfactant.
All three are non-ionic detergents and are good for cell lysis.
Nonidet-P40 (not the same as NP-40) is typically used for GST pulldown.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
Posted 20 March 2009 - 07:53 AM
some times when we are making RIPA lysis buffer we can either use 1% of Triton X-100 or 1% NP40....more or less they work the same...these two detergents almost break down everything in Eukaryotic cells....some times Sodium Deoxycholate is added to RIPA to guarantee break down of nuclei.
but Tween 20 is a little milder....it is a very common detergent for washing steps during western blot and ImmunoFluorescence experiments....it is also used for subcellular fractionation as its low amounts can only break down the cell membrane.
Posted 01 September 2011 - 08:24 AM
NP40=Ipegal from Sigma, just in case you need it.
Actually, Nonidet-P40 = IPEGAL
The full name of NP-40 is Tergitol-type NP-40, which is nonylphenoxypolyethoxylethanol
This whole terminology thing is very annoying.
Posted 01 September 2011 - 11:57 PM
I always had an alternate hypothesis....
Posted 03 September 2011 - 04:52 PM