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resuspend membrane pellet


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#1 genz

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Posted 19 March 2009 - 10:28 PM

Hi everyone,

I am working with a membrane protein. Fractionation involves a centrifugation step at 100 000 g for 1 hour. However, the pellet after centrifugation is so resilient to resuspension.

Can anyone suggest a good way to resuspend a membrane protein pellet after high speed spin?
Thanks inadvance

GE

#2 mdfenko

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Posted 20 March 2009 - 08:30 AM

you could try a dounce homogenizer.
talent does what it can
genius does what it must
i do what i get paid to do

#3 sudhakar mutyala

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Posted 24 April 2009 - 03:00 AM

Hi

Use mild denaturing agents Like Urea or GuCl in less conecntrations to solubilize the preoteins. Dialyse if possible


Regards
Sudhakar

Hi everyone,

I am working with a membrane protein. Fractionation involves a centrifugation step at 100 000 g for 1 hour. However, the pellet after centrifugation is so resilient to resuspension.

Can anyone suggest a good way to resuspend a membrane protein pellet after high speed spin?
Thanks inadvance

GE






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