Hi everyone,
I am working with a membrane protein. Fractionation involves a centrifugation step at 100 000 g for 1 hour. However, the pellet after centrifugation is so resilient to resuspension.
Can anyone suggest a good way to resuspend a membrane protein pellet after high speed spin?
Thanks inadvance
GE
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2 replies to this topic
#2
mdfenko
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Posted 20 March 2009 - 08:30 AM
you could try a dounce homogenizer.
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genius does what it must
i do what i get paid to do
#3
sudhakar mutyala
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Posted 24 April 2009 - 03:00 AM
Hi
Use mild denaturing agents Like Urea or GuCl in less conecntrations to solubilize the preoteins. Dialyse if possible
Regards
Sudhakar
Use mild denaturing agents Like Urea or GuCl in less conecntrations to solubilize the preoteins. Dialyse if possible
Regards
Sudhakar
genz, on Mar 19 2009, 10:28 PM, said:
Hi everyone,
I am working with a membrane protein. Fractionation involves a centrifugation step at 100 000 g for 1 hour. However, the pellet after centrifugation is so resilient to resuspension.
Can anyone suggest a good way to resuspend a membrane protein pellet after high speed spin?
Thanks inadvance
GE
I am working with a membrane protein. Fractionation involves a centrifugation step at 100 000 g for 1 hour. However, the pellet after centrifugation is so resilient to resuspension.
Can anyone suggest a good way to resuspend a membrane protein pellet after high speed spin?
Thanks inadvance
GE
