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plasmid copy number


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#1 somu

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Posted 19 March 2009 - 08:13 PM

Hiiii

I want to determine plasmid copy number in one popular strain of E.coli. Currently I am using fluoresence method in corelation with cfu/ml of the culture. It is a tediously long procedure as well as accuracy is not so good. I found huge variation every time.Can anybody please tell me some quick and accurate method of determination of plasmid copy number?

Thanks

#2 lactolee

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Posted 19 March 2009 - 09:50 PM

Hiiii

I want to determine plasmid copy number in one popular strain of E.coli. Currently I am using fluoresence method in corelation with cfu/ml of the culture. It is a tediously long procedure as well as accuracy is not so good. I found huge variation every time.Can anybody please tell me some quick and accurate method of determination of plasmid copy number?

Thanks



Real-time PCR will be helpful in this context. Maybe u can read the following journal:

Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria
Chai Lian Lee, Dave Siak Wei Ow, Steve Kah Weng Oh *
Journal of Microbiological Methods 65 (2006) 258 267

Lactolee

#3 somu

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Posted 20 March 2009 - 03:26 AM

Thank you.




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