2 replies to this topic

[somu]

Hiiii

I want to determine plasmid copy number in one popular strain of E.coli. Currently I am using fluoresence method in corelation with cfu/ml of the culture. It is a tediously long procedure as well as accuracy is not so good. I found huge variation every time.Can anybody please tell me some quick and accurate method of determination of plasmid copy number?

Thanks

[lactolee]

somu, on Mar 20 2009, 12:13 PM, said:

Hiiii

I want to determine plasmid copy number in one popular strain of E.coli. Currently I am using fluoresence method in corelation with cfu/ml of the culture. It is a tediously long procedure as well as accuracy is not so good. I found huge variation every time.Can anybody please tell me some quick and accurate method of determination of plasmid copy number?

Thanks

Real-time PCR will be helpful in this context.  Maybe u can read the following journal:

Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria
Chai Lian Lee, Dave Siak Wei Ow, Steve Kah Weng Oh *
Journal of Microbiological Methods 65 (2006) 258– 267

Lactolee

[somu]

Thank you.