I'm interested in determining if my protein is running as a dimer (or higher). I have a peptide antibody that works on denatured protein but when I ran a non-reducing gel, the antibody did not recognize the protein. I would like to be able to denature the protein in the (non-reducing) gel after it has run to determine if the protein is running as an oligomer. Does anyone know how I would go about denaturing the protein in the gel?
0
1 reply to this topic
#2
mdfenko
-
- Active Members
-
- 2,247 posts
an elder
78
Excellent
Excellent
Posted 20 March 2009 - 08:33 AM
you could try soaking in sds and 2-me (or dtt).
or you could try transferring then soaking in sds and 2-me to denature on the membrane.
or you could try transferring then soaking in sds and 2-me to denature on the membrane.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
