problem with Western blot loadig control
Posted 19 March 2009 - 01:37 PM
I just wanna ask a very basic question about the choice of loading control.
I want to detect an enzyme protein (estimate MW is around 30-45 KD) in bacteria with western blot, but I don't know what loading control could be used since according to my knowledge, actin, tubulin and GADDH is specific for eukaryotes.
I'll be very apprecitive of any suggestion.
Thanks a lot!
Posted 20 March 2009 - 07:32 AM
Prokaryotic origin of the actin cytoskeleton
FUSINITA VAN DEN ENT, LINDA A. AMOS & JAN LÖWE
Nature 413, 39-44 (6 September 2001)
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
Posted 23 March 2009 - 12:47 PM
However, I guess it'll be better to use the loading control that was commonly used by others.
Has anyone ever used a loading control for the quantitative western blot detection of the bacteria protein?
I am waiting for any useful suggestions.