Quick lipofectamine question
Posted 19 March 2009 - 12:40 PM
I am using a 24 well format, so I grow my cells in about .5ml of media in each well. Am I supposed to leave it or remove it and just add the ~100 ul of lipofectamine reagents by itself?
Posted 19 March 2009 - 01:17 PM
Posted 19 March 2009 - 10:00 PM
Posted 19 March 2009 - 11:09 PM
Posted 20 March 2009 - 01:35 AM
As an aside, further transfections on the now immortalised cells using lipofectamine2000 (same conc DNA and lipo as first transfection) in the presence of serum has not shown any uptake of plasmid
Posted 20 March 2009 - 06:26 AM
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
Posted 21 March 2009 - 10:36 PM
Posted 22 March 2009 - 09:03 AM