Anyone know how best to store viruses long term?
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Virus storage
Started by microgirl, Mar 19 2009 07:30 AM
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#2
leelee
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Posted 19 March 2009 - 06:29 PM
At -80C or in liquid nitrogen
#3
microgirl
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Posted 20 March 2009 - 04:50 AM
leelee, on Mar 19 2009, 06:29 PM, said:
At -80C or in liquid nitrogen
Do you put it in glycerol like bacterial cultures?
#4
scolix
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Posted 21 March 2009 - 10:38 PM
no glycerol.
Just have them in what ever buffer they are in and freeze it in small aliquots. so you dont have to refreeze many times. But this also depends on the virus.
Just have them in what ever buffer they are in and freeze it in small aliquots. so you dont have to refreeze many times. But this also depends on the virus.
#5
Wolverena
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Posted 22 March 2009 - 02:04 PM
microgirl, on Mar 20 2009, 08:50 AM, said:
leelee, on Mar 19 2009, 06:29 PM, said:
At -80C or in liquid nitrogen
Do you put it in glycerol like bacterial cultures?
Sometimes people use DMSO....
"You can give somebody a book on 'How to ride bike' and then test that person on that knowledge. Even if that person gets an "A", it doesn't mean that he or she can ride a bike."
---this is what I am telling myself when I get a bad grade....as long as you don't loose your passion, you'll be fine.....V
---this is what I am telling myself when I get a bad grade....as long as you don't loose your passion, you'll be fine.....V
#6
three
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Posted 29 March 2009 - 06:36 AM
Is it ok to store the virus at -80 in the culture medium?
#7
pito
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Posted 29 March 2009 - 06:44 AM
three, on Mar 29 2009, 04:36 PM, said:
Is it ok to store the virus at -80 in the culture medium?
Depends on the culture media.. pH etc...
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.
#8
three
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Posted 30 March 2009 - 09:20 AM
What buffer do you recommend? Is PBS ok?
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Wolverena
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Posted 30 March 2009 - 09:48 AM
three, on Mar 30 2009, 01:20 PM, said:
What buffer do you recommend? Is PBS ok?
a recommended buffer is SM buffer [from Sambrook]:
per liter:
NaCl = 5.8g
MgSO8 X 7 H2O = 2g
1M Tris-HCl (pH 7.5) = 50ml
2% (w/v) gelatin solution = 5ml
H2O to 1 liter
sterilize by autoclaving and than dispense into aliquots. Store at RT.
"You can give somebody a book on 'How to ride bike' and then test that person on that knowledge. Even if that person gets an "A", it doesn't mean that he or she can ride a bike."
---this is what I am telling myself when I get a bad grade....as long as you don't loose your passion, you'll be fine.....V
---this is what I am telling myself when I get a bad grade....as long as you don't loose your passion, you'll be fine.....V
#10
Curtis
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Posted 12 September 2012 - 09:30 PM
We keep in PBS or TEN buffer and store in -80.
