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No beta actin results at all???


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#1 c.jakobsen

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Posted 19 March 2009 - 06:04 AM

Hi all,

We are just setting up a qRT-PCR system and have only run a few times. Both times we got absolutely no response from beta actin. We are running TaqMan on an AB 7900HT system. Does anyone have any idea why this would be, besides contamination of the sample?

Thanks,

C.jakobsen

#2 tea-test

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Posted 19 March 2009 - 07:44 AM

This can have many reasons. Maybe your RNA was degraded (did you check on gel?) or you forgot to add RT enzyme or you analyzed the wrong fluorescence channel on the machine eg. FAM instead of VIC or you put far too much template in the reaction, primer/probes of the wrong organism :( or wrong primer/probe design....

We would need more information about what exactly you are doing.
tea-test: The artist formerly known as Ned Land




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