sanku, on 10 February 2011 - 04:49 PM, said:
Can anybody let me know whether 293T cells are attached (adherent) cells. Is there any need to coat the flask with Poly lysine for these cells. How many passage can be done with those.
thanks in advance
The 293T (cultured in DMEM w/ FCS) I have used were adherent, but we used flasks that were already treated, so we didn't need to do anything to the flask. If culturing in different media you might have different levels of adhesion - for example using a serum free media seems to make them less attached and take on a different morphology.