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cell culture guideline from abcam


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5 replies to this topic

#1 shs

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Posted 18 March 2009 - 05:43 PM

Hi
I hope this will help all of us

Best wishes
shirri

Attached Files


Science is What We Need To Understan The Life

#2 pcrman

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Posted 18 March 2009 - 07:42 PM

Thanks for sharing. It is very useful.

#3 respirator

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Posted 13 June 2009 - 04:20 AM

that's helpful. thank you

I have a question?

I am doing sputum processing and when I finhsed from the haemocytometer count, i have to centrifuge the the filtrate of the sample and then remove the supernatant and leaving the cell pellet, I need to know how THEN resuspend the cell pellet in PBS and then adjust the cells/volume to give me 0.5 million for preparation of cytospin slides!

Thank you

#4 MANISH

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Posted 19 August 2010 - 02:35 AM

Thank for this pdf.
Can you tell me the detailed process and requirements for the U937 cell culture?
Plzzzzzz reply soon

Thank you,

#5 sanku

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Posted 10 February 2011 - 04:49 PM

Can anybody let me know whether 293T cells are attached (adherent) cells. Is there any need to coat the flask with Poly lysine for these cells. How many passage can be done with those.
thanks in advance

#6 Andrew C

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Posted 17 May 2011 - 02:41 PM

Can anybody let me know whether 293T cells are attached (adherent) cells. Is there any need to coat the flask with Poly lysine for these cells. How many passage can be done with those.
thanks in advance


The 293T (cultured in DMEM w/ FCS) I have used were adherent, but we used flasks that were already treated, so we didn't need to do anything to the flask. If culturing in different media you might have different levels of adhesion - for example using a serum free media seems to make them less attached and take on a different morphology.




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