Agarose gel cutting
Posted 18 March 2009 - 07:30 AM
Posted 18 March 2009 - 08:11 AM
I am terrible at cutting my dna from an agarose gel. I dice it up pretty much and still have large pieces of agarose without dna. Do you have any advice for cutting up the gel with minimal agarose for purification? Thanks
What do you use to cut your gel?
I use a fresh scalpel blade. But I only press down into the gel. I don't drag the blade in a cutting motion to avoid damaging the glass surface of the transluminator.
Posted 18 March 2009 - 10:39 AM
Posted 18 March 2009 - 12:01 PM
Practice helps a lot, I was quite bad too, therefore I cut several times DNA ladders not needed anymore.
Here some just mark with a first "preliminary" cut the area they need and then cut on the unilluminated surface besides the glass.
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 19 March 2009 - 12:12 AM
Posted 14 May 2009 - 10:00 PM
Posted 21 May 2009 - 12:21 AM
Posted 21 May 2009 - 03:35 AM
to make my life easier, i make use of an transilluminator without UV that still allows me to view my gel and cut them without the lights being on.
Are u saying that u don't use UV to view Etbr stained DNA??
Posted 21 May 2009 - 04:21 PM