problems with detecting protein expression
Posted 18 March 2009 - 05:07 AM
in order to detect the protein expression in cell, either in cytoplasm or in the nucleus,
anyone could help me this.
i have already find out the method by using flurosence marker and observe with confocol,
but i have no confocol microscopy in lab, any other "classical" method to proof the protein either express in cytoplasm or in nucleus?
Posted 18 March 2009 - 03:01 PM
Posted 02 April 2009 - 11:31 PM
Micro-organisms don't have a nucleus... and organisms that do have a nucleus don't have ribosomes in their nucleus. So are you interested in the final location of the proteins, rather than where they are expressed?
i am doing virus replication, and try to find out where the protein are in the cell by using cell culture.
Posted 05 April 2009 - 09:14 PM