We differentiate stem cells into cardiomyocites and now we want to study the methylation profile of some genes during differentiation. I have some pieces of human heart and I would like to use it as positive control of DNA methylation.
Which protocol of DNA extraction do you guys suggests in which DNA is suitable to BSP studies?
We have huge pieces of heart like half ventricule or atrium. They are frozen at liquid nitrogen.
Edited by dvddecarvalho, 18 March 2009 - 04:23 AM.