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#1 aarthy mayil vahanan

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Posted 18 March 2009 - 03:05 AM

My primers ARE FORMING primer- dimers. Giving me a very hard time. :D So i thought i can use DMSO. Sud i dilute my DMSO. ( in the bottle label its given 99% DMSO) For a 50 microl reaction how much diluted(?) DMSO should i add. i had used ApE A plasmid editor for designing my primer. Is the s/w good? My fwd primer length is 33 bp and reverse 30 bp including the restriction enzyme site region i had added.I could not avoid the length.

#2 merlav

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Posted 18 March 2009 - 05:33 AM

o.5ÁL/50ÁL of reaction
Science without religion is lame, religion without science is blind.
Albert Einstein

I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale.
Marie Curie




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