Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

yeast two hybrid


  • Please log in to reply
1 reply to this topic

#1 Bomber

Bomber

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 52 posts
0
Neutral

Posted 17 March 2009 - 12:15 PM

Hi all,

if you do a Y2H screen with human or mouse libraries, would you think the detected iinteractions are species specific?
Assuming you find a potential binding factor in a human cDNA library with a human bait, how likely is it that this interaction can also be found e.g in mouse?
I guess one would do some conservation studies to see how well the analysed proteins are conserved - is there anything known more generally about this?
In publications one usually find protein interactions for one species 'only'.

Thanks for any ideas.
Best wishes

#2 Dr Teeth

Dr Teeth

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 221 posts
1
Neutral

Posted 18 March 2009 - 05:37 AM

Hi all,

if you do a Y2H screen with human or mouse libraries, would you think the detected iinteractions are species specific?
Assuming you find a potential binding factor in a human cDNA library with a human bait, how likely is it that this interaction can also be found e.g in mouse?
I guess one would do some conservation studies to see how well the analysed proteins are conserved - is there anything known more generally about this?
In publications one usually find protein interactions for one species 'only'.

Thanks for any ideas.
Best wishes


As you said, it most likely depends on the homology between the human and mouse proteins. I would not assume that these interactions were maintained, but most times they likely would be. It is best to test human proteins against human cDNA libraries.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.