1 reply to this topic

[Bomber]

Hi all,

if you do a Y2H screen with human or mouse libraries, would you think the detected iinteractions are species specific?
Assuming you find a potential binding factor in a human cDNA library with a human bait, how likely is it that this interaction can also be found e.g in mouse?
I guess one would do some conservation studies to see how well the analysed proteins are conserved - is there anything known more generally about this?
In publications one usually find protein interactions for one species 'only'.

Thanks for any ideas.
Best wishes

[Dr Teeth]

Bomber, on Mar 17 2009, 04:15 PM, said:

Hi all,

if you do a Y2H screen with human or mouse libraries, would you think the detected iinteractions are species specific?
Assuming you find a potential binding factor in a human cDNA library with a human bait, how likely is it that this interaction can also be found e.g in mouse?
I guess one would do some conservation studies to see how well the analysed proteins are conserved - is there anything known more generally about this?
In publications one usually find protein interactions for one species 'only'.

Thanks for any ideas.
Best wishes

As you said, it most likely depends on the homology between the human and mouse proteins.  I would not assume that these interactions were maintained, but most times they likely would be.  It is best to test human proteins against human cDNA libraries.