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what is meant by passages


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3 replies to this topic

#1 youyou

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Posted 17 March 2009 - 09:07 AM

Hi

i wanted to know exactly what is meant by passage in cell culture and how many passages can be max. done for Nalm6 cells ? :(

#2 Kami23

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Posted 17 March 2009 - 09:29 AM

Hi

i wanted to know exactly what is meant by passage in cell culture and how many passages can be max. done for Nalm6 cells ? :(



The term passage means to split the cells down so they dont become overpopulated. In my experience this can be done 9 or 10 times before the cells begin to become useless but for your specific strain I dont know :) I used COS-7 and another more delicate type of cell (the name escapes me!)

#3 Dr Teeth

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Posted 17 March 2009 - 10:11 AM

Hi

i wanted to know exactly what is meant by passage in cell culture and how many passages can be max. done for Nalm6 cells ? :(



The term passage means to split the cells down so they dont become overpopulated. In my experience this can be done 9 or 10 times before the cells begin to become useless but for your specific strain I dont know :) I used COS-7 and another more delicate type of cell (the name escapes me!)


Unless you are using primary cells or have a very sensitive endpoint, 9 or 10 passages shouldn't be the limit. For my system, various dose response parameters (like Vmax and EC50) are consistent oftentimes beyond 200 passages for COS-7, CV-1, and U2OS cells.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#4 labrat612

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Posted 17 March 2009 - 12:48 PM

I like to just keep my cells around for 2 months or 24 passages, whichever should come first.




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