3 replies to this topic

[youyou]

Hi

i wanted to know exactly what is meant by passage in cell culture and how many passages can be max. done for Nalm6 cells  ?  

[Kami23]

youyou, on Mar 17 2009, 05:07 PM, said:

Hi

i wanted to know exactly what is meant by passage in cell culture and how many passages can be max. done for Nalm6 cells  ?  

The term passage means to split the cells down so they dont become overpopulated.  In my experience this can be done 9 or 10 times before the cells begin to become useless but for your specific strain I dont know I used COS-7 and another more delicate type of cell (the name escapes me!)

[Dr Teeth]

Kami23, on Mar 17 2009, 01:29 PM, said:

youyou, on Mar 17 2009, 05:07 PM, said:

Hi

i wanted to know exactly what is meant by passage in cell culture and how many passages can be max. done for Nalm6 cells  ?  

The term passage means to split the cells down so they dont become overpopulated.  In my experience this can be done 9 or 10 times before the cells begin to become useless but for your specific strain I dont know I used COS-7 and another more delicate type of cell (the name escapes me!)

Unless you are using primary cells or have a very sensitive endpoint, 9 or 10 passages shouldn't be the limit.  For my system, various dose response parameters (like Vmax and EC50) are consistent oftentimes beyond 200 passages for COS-7, CV-1, and U2OS cells.

[labrat612]

I like to just keep  my cells around for 2 months or 24 passages, whichever should come first.