Ideal incubation time
Posted 17 March 2009 - 04:34 AM
Can someone enlighten me on why the incubation time for ampicillin plates should not be more than 16 hours?
I have been told, ideally, incubation time should be about 12-16 hours, but why when it passes the 16th hour mark, it is not ideal anymore?
Posted 17 March 2009 - 03:39 PM
Posted 17 March 2009 - 05:38 PM
If you are doing colony PCR, old colonies don't amplify well. Once the colony becomes too big, the cells become stressed and start to increase production of lipopolysaccarides. Which seem to interfere with the PCR reaction.
You can grow your colonies longer than 16hr if you drop the incubation temperature.