Our lab works on tadpoles and we do RNA extraction from them.Offlate, the A260/A280 ratios have been between 1.42 and 1.56 unlike our prevoius extractions wherein we were getting a ratio of 1.6.
An 0.8% agarose gel showed three bands with a third band being very prominent.We noticed that when the ratios became around 1.45, the third band appears very thick.We use Trizol from Invitrogen for RNA extraction.Isopropyl alcohol and chloroform from Sigma.All the steps are carried out in cold(4C-8C).
Can anyone help us as to why this is occurring.
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appearance RNA on 0.8% gel
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