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Design degenerate primers to clone unknown gene

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#1 sagar



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Posted 17 March 2009 - 02:26 AM

hi everyone
Im doing a project where i have to isolate a particular gene from plant and im not very much sure how to proceed after isolation of the mrnas. i need to search for the conserved domain for the protein and then design primer.can anyone help me go in a correct approach like how to use ncbi ?im confused.
thanks in advance

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