how to remove a restricted endonuclease site
Posted 16 March 2009 - 06:21 PM
My question is whether this point mutation could really eliminate the RE recognization and digestion. Someone claimed they perform point mutation in RE sites, however, protein still is digested by RE. Whether the mutated site design has some tricks I don't kown. Any people any experiences were welcome.
Posted 16 March 2009 - 06:30 PM
Posted 16 March 2009 - 06:32 PM
Posted 16 March 2009 - 06:39 PM
For PstI, a single bp change to its restriction site would be sufficient to knock out the site.
This strategy will work fine. But the DNA is not a protein(!).
Thank you very much.