Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Control vehicle treatment in cells


  • Please log in to reply
2 replies to this topic

#1 dsk

dsk

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 16 March 2009 - 05:32 PM

Hi,

I read about control vehicle treatment (like ethanol or DMSO) in cells, but never understood what exactly it means. Do i treat the same volume of ethanol or DMSO as present in my ligand? Please clarify.

#2 little mouse

little mouse

    Missele, the little mouse

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 172 posts
2
Neutral

Posted 17 March 2009 - 02:25 AM

exactly.
if you add 200 ÁL of your ligand, in DMSO (or PBS, water, ethanol...), then you need to do a control where you add 200ÁL of DMSO (or PBS, water, ethanol...) alone

#3 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,659 posts
393
Excellent

Posted 17 March 2009 - 03:43 PM

... to continue "little mouse's" post...

so that you can distinguish the effect of the treatment from the carrier/vehicle effect.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.