Control vehicle treatment in cells
Posted 16 March 2009 - 05:32 PM
I read about control vehicle treatment (like ethanol or DMSO) in cells, but never understood what exactly it means. Do i treat the same volume of ethanol or DMSO as present in my ligand? Please clarify.
Posted 17 March 2009 - 02:25 AM
if you add 200 ÁL of your ligand, in DMSO (or PBS, water, ethanol...), then you need to do a control where you add 200ÁL of DMSO (or PBS, water, ethanol...) alone
Posted 17 March 2009 - 03:43 PM
so that you can distinguish the effect of the treatment from the carrier/vehicle effect.