Hi,
I read about control vehicle treatment (like ethanol or DMSO) in cells, but never understood what exactly it means. Do i treat the same volume of ethanol or DMSO as present in my ligand? Please clarify.
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#2
little mouse
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Missele, the little mouse
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Neutral
Neutral
Posted 17 March 2009 - 02:25 AM
exactly.
if you add 200 µL of your ligand, in DMSO (or PBS, water, ethanol...), then you need to do a control where you add 200µL of DMSO (or PBS, water, ethanol...) alone
if you add 200 µL of your ligand, in DMSO (or PBS, water, ethanol...), then you need to do a control where you add 200µL of DMSO (or PBS, water, ethanol...) alone
#3
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 17 March 2009 - 03:43 PM
... to continue "little mouse's" post...
so that you can distinguish the effect of the treatment from the carrier/vehicle effect.
so that you can distinguish the effect of the treatment from the carrier/vehicle effect.
