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substractive hybridization


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2 replies to this topic

#1 novagen

novagen

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Posted 16 March 2009 - 09:19 AM

Hi all,
Does anyone have experience on workig with substractive hybridization,clontech. i would like to clarify few doubts with them
Thanks a million tons
Service to man is service to God

#2 erne

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Posted 16 March 2009 - 01:12 PM

I have some experience. What's your doubts?

Hi all,
Does anyone have experience on workig with substractive hybridization,clontech. i would like to clarify few doubts with them
Thanks a million tons



#3 novagen

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Posted 19 March 2009 - 06:57 AM

Hi
How to analyse adapter ligation?
as per the kit procedure I did pcr with primer1 and housekeeping gene but I could visualize two high mol.w bands how do i analyze this result??
thanks a lot
Service to man is service to God




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