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miRNA Northern Blot gel


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4 replies to this topic

#1 stardust

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Posted 16 March 2009 - 06:13 AM

Hi,

I have a quick question. Does anyone know, if I can prepare a 15 % TBE Urea gel today and then store it in the fridge for using it tomorrow? How would you store it?

Stardust

#2 pcrman

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Posted 17 March 2009 - 09:56 PM

Wrap the gel in plastic food wrap and store O/N at 4C or room temperature should be fine.

#3 stardust

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Posted 18 March 2009 - 02:05 AM

Thank you, I'll try it

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#4 stardust

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Posted 18 March 2009 - 09:15 AM

Just an update:
I store it at 4C (maybe the fridge is colder ?) and the urea seems to have precipitated...I guess RT would have been better...I'll try next time

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#5 RNA woman

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Posted 13 April 2009 - 09:06 PM

I try before and I think sotrage in 4 drgree in 1XTBE buffer is good O/Nor even up to one week. But prerun step is a must for 20min 140V.




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