Hi,
I have a quick question. Does anyone know, if I can prepare a 15 % TBE Urea gel today and then store it in the fridge for using it tomorrow? How would you store it?
Stardust
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miRNA Northern Blot gel
Started by stardust, Mar 16 2009 06:13 AM
4 replies to this topic
#2
pcrman
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Excellent
Excellent
Posted 17 March 2009 - 09:56 PM
Wrap the gel in plastic food wrap and store O/N at 4C or room temperature should be fine.
#3
stardust
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Posted 18 March 2009 - 02:05 AM
Thank you, I'll try it
Stardust
Stardust
#4
stardust
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Posted 18 March 2009 - 09:15 AM
Just an update:
I store it at 4°C (maybe the fridge is colder ?) and the urea seems to have precipitated...I guess RT would have been better...I'll try next time
Stardust
I store it at 4°C (maybe the fridge is colder ?) and the urea seems to have precipitated...I guess RT would have been better...I'll try next time
Stardust
#5
RNA woman
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Posted 13 April 2009 - 09:06 PM
I try before and I think sotrage in 4 drgree in 1XTBE buffer is good O/Nor even up to one week. But prerun step is a must for 20min 140V.
