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Limitations of Animal Tissue Cultures


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11 replies to this topic

#1 QQ_lin

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Posted 16 March 2009 - 04:46 AM

Animal tissue cultures can help to create many possibilities in medical advancement for now and in the future. Besides the ethics problems that
encountered many scientists, what are the other major limitations that scientists will face when performing animal tissue cultures especially for medical purposes such as organ transplantation, etc. ? :)

#2 cotchy

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Posted 16 March 2009 - 08:14 AM

This is an interesting question, even possibly a students college assignment?

So maybe you should try the old fashion way of answering a question by doing some research and reading some books for yourself.

Maybe then you can leave a reply telling us all the answer.

#3 QQ_lin

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Posted 16 March 2009 - 06:49 PM

This is an interesting question, even possibly a students college assignment?

So maybe you should try the old fashion way of answering a question by doing some research and reading some books for yourself.

Maybe then you can leave a reply telling us all the answer.



Actually I am now trying my best to find the possible answers. I also hope that I can share the answers that I will get with all of you very soon. Just want to post it here so that we all can think about it. Thanks for your comments. I appreciate it. :)

#4 bob1

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Posted 17 March 2009 - 03:41 PM

think about patient response, cell replication etc.

#5 QQ_lin

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Posted 18 March 2009 - 05:58 AM

think about patient response, cell replication etc.




Thanks for your suggestions. I am still in the progress of searching the best answers. :D

#6 QQ_lin

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Posted 28 March 2009 - 07:43 PM

Hello friends,

I have figured out some of the limitations in animal tissue culture for medical advancement which as listed as below:

1. Cross-contamination of the cell lines
2. Instability of the continuous cell lines due to chromosomal instability
3. The differences between in vitro and in vivo system during drug testing and organ transplantation
4. Origin of the explants

Do you think the limitations stated above can be totally overcome in the next future with all the developments of advance technology and techniques? :lol:

#7 bob1

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Posted 29 March 2009 - 03:53 PM

Good work, those were some of the issues associated with the original question.

I would add patient responses to the list, especially rejection of the tissue/cells implanted. I would also add the risk of generating some foreign tumour type - many cell lines are very very aggressive in culture and are likely to be so in patients also, so if they escape the implant, they could cause cancers, especially if the patient is on immuno-suppressant drugs. There is also the risk of infection from the implants, many cell cultures have been transformed with/by viruses, and some even still shed live virus, though this can be easily tested for.

With regards to overcoming the problems - the answer is no, we can't over come the genetic instability of cells yet, and it isn't really feasible in the near (20+ years) future.

#8 QQ_lin

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Posted 30 March 2009 - 12:31 PM

Good work, those were some of the issues associated with the original question.

I would add patient responses to the list, especially rejection of the tissue/cells implanted. I would also add the risk of generating some foreign tumour type - many cell lines are very very aggressive in culture and are likely to be so in patients also, so if they escape the implant, they could cause cancers, especially if the patient is on immuno-suppressant drugs. There is also the risk of infection from the implants, many cell cultures have been transformed with/by viruses, and some even still shed live virus, though this can be easily tested for.

With regards to overcoming the problems - the answer is no, we can't over come the genetic instability of cells yet, and it isn't really feasible in the near (20+ years) future.



Thanks for your information. I appreciate a lots. I would like to find out more on genetic instability of the cells. Do you have any resources regarding this issue that you can recommend to me for my additional readings?

Thanks. :P

#9 bob1

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Posted 30 March 2009 - 04:12 PM

Look out for papers regarding the end-replication problem/telomere shortening and the mechanisms involved.

#10 QQ_lin

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Posted 31 March 2009 - 06:28 AM

Look out for papers regarding the end-replication problem/telomere shortening and the mechanisms involved.



Thanks! :)

#11 respirator

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Posted 13 June 2009 - 04:25 AM

I have a question guys?

I am doing sputum processing and when I finhsed from the haemocytometer count, i have to centrifuge the the filtrate of the sample and then remove the supernatant and leaving the cell pellet, I need to know how THEN resuspend the cell pellet in PBS and then adjust the cells/volume to give me 0.5 million for preparation of cytospin slides!

Thank you

#12 Dr.rania

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Posted 24 November 2009 - 02:46 AM

i need your help please , tell me the method
how i make study about new chemotheraputic agent effect on cells of paranasal sinuses and fungal cells
please help quickly
thank you in advance




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