HI, i would like to ask about is it feasible to mix 2 tubes of pcr product (different mgcl2 concentration) for purification from gel and then for sequencing?
the products have the same size (same position).
0
4 replies to this topic
#2
T C
-
- Active Members
-
- 277 posts
Veteran
0
Neutral
Neutral
Posted 16 March 2009 - 05:29 AM
Yeah
Its okay, no problem.
Best
TC
Its okay, no problem.
Best
TC
#3
gebirgsziege
-
- Active Members
-
- 392 posts
I like fungi
23
Excellent
Excellent
Posted 16 March 2009 - 05:29 AM
same gene and organism????
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)
#4
T C
-
- Active Members
-
- 277 posts
Veteran
0
Neutral
Neutral
Posted 16 March 2009 - 05:43 AM
Yeah........Gebirgsziege has a point.....its got to be teh same product from the same gene and organism.........from yr post I took it for granted that the only difference between the 2 tubes if magnesium ion concentration.
Best
TC
Best
TC
gebirgsziege, on Mar 16 2009, 06:59 PM, said:
same gene and organism????
#5
aaron86
-
- Members
-
- 2 posts
member
0
Neutral
Neutral
Posted 16 March 2009 - 05:59 AM
THX for all the replies, ya, it is from the same gene and same organism. only the magnesium chloride concentrations are different. really thz alot!
