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sequencing problem after pcr


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4 replies to this topic

#1 aaron86

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Posted 16 March 2009 - 03:31 AM

HI, i would like to ask about is it feasible to mix 2 tubes of pcr product (different mgcl2 concentration) for purification from gel and then for sequencing?
the products have the same size (same position).

#2 T C

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Posted 16 March 2009 - 05:29 AM

Yeah

Its okay, no problem.

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TC

#3 gebirgsziege

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Posted 16 March 2009 - 05:29 AM

same gene and organism????
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

#4 T C

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Posted 16 March 2009 - 05:43 AM

Yeah........Gebirgsziege has a point.....its got to be teh same product from the same gene and organism.........from yr post I took it for granted that the only difference between the 2 tubes if magnesium ion concentration.

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TC

same gene and organism????



#5 aaron86

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Posted 16 March 2009 - 05:59 AM

THX for all the replies, ya, it is from the same gene and same organism. only the magnesium chloride concentrations are different. really thz alot!




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