Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Detecting glucose in reactions with OD


  • Please log in to reply
5 replies to this topic

#1 longer

longer

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 16 March 2009 - 02:46 AM

Please if one can help by giving me method for detecting glucose By OD without converting glucose to any products
for examples: without using glusoe oxidase and then peroxidase
and if possible to get out the glucose concentration
please help me


#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,811 posts
136
Excellent

Posted 16 March 2009 - 08:17 AM

do you want to detect it in free solution or from some other source?

will there be any other sugars present?

in lc you can detect eluted sugars at 232nm or with a refractive index detector.
talent does what it can
genius does what it must
i do what i get paid to do

#3 longer

longer

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 17 March 2009 - 12:17 AM

do you want to detect it in free solution or from some other source?

will there be any other sugars present?

in lc you can detect eluted sugars at 232nm or with a refractive index detector.

I want to detect the glucose converted from another sugar by new enzymatic reaction

#4 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,811 posts
136
Excellent

Posted 17 March 2009 - 10:57 AM

you will not be able to identify a specific sugar unless you separate them on a calibrated column.

you will not be able to differentiate the sugars by od alone.
talent does what it can
genius does what it must
i do what i get paid to do

#5 DRT

DRT

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 162 posts
7
Neutral

Posted 17 March 2009 - 02:46 PM

If glucose is the product of your reaction why are you worried about destroying/changing it during detection?

#6 longer

longer

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 23 March 2009 - 01:49 AM

If glucose is the product of your reaction why are you worried about destroying/changing it during detection?


because of it require long time i need short step with short time for HTS




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.