Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

what is co transformation scientificaly ?


  • Please log in to reply
1 reply to this topic

#1 dawood

dawood

    member

  • Active Members
  • Pip
  • 4 posts
0
Neutral

Posted 15 March 2009 - 11:16 PM

Everybody Hi ,
Actually my experiment was cotransformation for Top10 , but my Professor told me i have to prepare a competent cell harboring one plasmid then i have to do a single transformation, here i got much more clones in plate than in cotransformation .
Can anybody tell me what is the scientific reason for the difference in enumerated clones betwwen them ?
Thank you
Dawood

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,721 posts
399
Excellent

Posted 22 March 2009 - 03:49 PM

Presuming that what you have been told to do is either
1) Transform both plasmids at once
or
2) Transform the plasmids one after the other

And the question is why is there a higher number of clones produced when transforming the second plasmid for (2) above.

The answer is that if you try and transform and have different selective markers on each plasmid (e.g. ampicillin on one and kanamycin on the other), the number of bacteria that will take up both is limited. However, if you do them sequentially as in (2), "all" the bacteria being transformed already have the first plasmid there, so it is just like transforming one plasmid.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.