Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

What is the difference between cotransformation and (single transformation to th

  • Please log in to reply
2 replies to this topic

#1 dawood



  • Active Members
  • Pip
  • 4 posts

Posted 15 March 2009 - 11:11 PM

Every body Hi ,
Actually my experiment was cotransformation for Top10 , but my Professor told me i have to prepare competent cell harboring one plasmid then i have to do single transformation, here i got much more clones in plate than in cotransformation .
Can anybody tell me what is the scientific reason for the difference in enumerated clones betwwen them ?
Thank you

#2 molgen



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 167 posts

Posted 16 March 2009 - 01:54 AM

I've never dun cotransformation but as I understand it, in one reaction of transformation you put 2 plasmids.
From my experience with transfections, the number of cells that accept both plasmids is small.
On the other hand, if you do 2 separate transformations, the yield is higher.

#3 isbow



  • Active Members
  • Pip
  • 10 posts

Posted 16 March 2009 - 04:39 AM

I had absolutly no problems with cotransformation, you maybe should take a little bit more plasmid DNA. The good thing in first putting in one plasmid and then transforming these cells with another plasmid might be that you don't have to check for both plasmids later if you don't have different selection markers. I needed the cells completely fresh cotransformed because I worked with a bacterial two hybrid system - therefore I can say that interaction activity decreases a lot when you transform them in two steps.

Hope this helps! :)

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.