What is the difference between cotransformation and (single transformation to th
Posted 15 March 2009 - 11:11 PM
Actually my experiment was cotransformation for Top10 , but my Professor told me i have to prepare competent cell harboring one plasmid then i have to do single transformation, here i got much more clones in plate than in cotransformation .
Can anybody tell me what is the scientific reason for the difference in enumerated clones betwwen them ?
Posted 16 March 2009 - 01:54 AM
From my experience with transfections, the number of cells that accept both plasmids is small.
On the other hand, if you do 2 separate transformations, the yield is higher.
Posted 16 March 2009 - 04:39 AM
Hope this helps!