4 replies to this topic

[cmel]

What is the general consensus? Do you use filter pipette tips or just autoclave regular pipette tips for tissue culture.

I realize that filter tips will obviously be better for reducing the chance of contamination from pipettes etc but, they are so $!

[CKtong]

cmel, on Mar 16 2009, 02:17 PM, said:

What is the general consensus? Do you use filter pipette tips or just autoclave regular pipette tips for tissue culture.

I realize that filter tips will obviously be better for reducing the chance of contamination from pipettes etc but, they are so $!

i think not neccessary to use filter tips( as you said, it is expensive). Jz use normal tips(either a pre-steril or autoclave). We have been use that for years(primary or cell line). What matter much is the techniques.

[little mouse]

most often I use regular tips.
I only use filter tips when I culture intracellular bacteria, or when I know I have quite some risk to make cross contamination (with potential mycoplasma infected cells in 96 well plates for example)

[memo]

Normally use dry-heated sterile tips instead of filter tips!

[labrat612]

I totally agree with little mouse. I'll use regular sterile tips and only change to filter tips if I suspect something strange or dealing with something that could potentially contaminate my cultures.