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filter tips or regular tips for tissue culture?


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4 replies to this topic

#1 cmel

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Posted 15 March 2009 - 10:17 PM

What is the general consensus? Do you use filter pipette tips or just autoclave regular pipette tips for tissue culture.

I realize that filter tips will obviously be better for reducing the chance of contamination from pipettes etc but, they are so $!

#2 CKtong

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Posted 16 March 2009 - 02:19 AM

What is the general consensus? Do you use filter pipette tips or just autoclave regular pipette tips for tissue culture.

I realize that filter tips will obviously be better for reducing the chance of contamination from pipettes etc but, they are so $!


i think not neccessary to use filter tips( as you said, it is expensive). Jz use normal tips(either a pre-steril or autoclave). We have been use that for years(primary or cell line). What matter much is the techniques.

#3 little mouse

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Posted 16 March 2009 - 07:01 AM

most often I use regular tips.
I only use filter tips when I culture intracellular bacteria, or when I know I have quite some risk to make cross contamination (with potential mycoplasma infected cells in 96 well plates for example)

#4 memo

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Posted 17 March 2009 - 07:28 AM

Normally use dry-heated sterile tips instead of filter tips!

#5 labrat612

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Posted 17 March 2009 - 12:50 PM

I totally agree with little mouse. I'll use regular sterile tips and only change to filter tips if I suspect something strange or dealing with something that could potentially contaminate my cultures.




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