3 replies to this topic

[Snoopy]

Hello,
I try to differentiate sh-sy5y neuroblastoma cells with 10 microM without that much success.

I want to know at which density you seed them for differentiation 'couse i use 20000/cm2 and it might be too much.

what else could i do to make them be more differentiated.

Waiting for your answer

snoopy  

[labrat612]

What agent were you using to differentiate? I've typically used retinoic acid.

[Snoopy]

I use 10 micromolar of retinoic acid with retinoic acid in full medium (10% Fetal calf serum)

[scolix]

Snoopy, on Mar 15 2009, 03:25 PM, said:

Hello,
I try to differentiate sh-sy5y neuroblastoma cells with 10 microM without that much success.

I want to know at which density you seed them for differentiation 'couse i use 20000/cm2 and it might be too much.

what else could i do to make them be more differentiated.

Waiting for your answer

snoopy  

try to add some neurotrophic factors like NGF or GDNF to promote neurite differentiation.