hi
i have a curious question.how do we design primers for a gene? is it just including the sequences at the 5' and 3' end of the cds of the gene?
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#2
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Posted 14 March 2009 - 09:50 PM
Yeah and engineering sites, getting it into the right frame to fit in the vector and code for say a tag along with yr protein.
sagar, on Mar 15 2009, 12:13 AM, said:
hi
i have a curious question.how do we design primers for a gene? is it just including the sequences at the 5' and 3' end of the cds of the gene?
i have a curious question.how do we design primers for a gene? is it just including the sequences at the 5' and 3' end of the cds of the gene?
#3
molgen
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Posted 15 March 2009 - 03:05 AM
It really depends on what you want to do.
Expression, quantification, mutation…..
Expression, quantification, mutation…..
#4
Curtis
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Posted 15 March 2009 - 07:11 AM
sagar, on Mar 14 2009, 10:43 AM, said:
hi
i have a curious question.how do we design primers for a gene? is it just including the sequences at the 5' and 3' end of the cds of the gene?
i have a curious question.how do we design primers for a gene? is it just including the sequences at the 5' and 3' end of the cds of the gene?
primer design it's a long story, don't expect to learn it from our replies.....you need to find info on protocol categories at protocol online website.
