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freezing of digitonin solutions


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#1 yobou

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Posted 14 March 2009 - 12:03 AM

Dear All
I would like to know if it is ok to store prepared digitonin solutions at - 20oC and re-using them after thawing?
thanks

#2 klinmed

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Posted 14 March 2009 - 12:26 AM

Dear All
I would like to know if it is ok to store prepared digitonin solutions at - 20oC and re-using them after thawing?
thanks


Invitrogen supply 5% digitonin solution and recommend storage at 4 oC. Your material should be stable at this temp. Check their website if you need more info. Storage at -20 oC should be fine.

#3 Curtis

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Posted 14 March 2009 - 07:26 AM

Do you mean Digitonin solution for cell lysis?

I keep mine at 4 degrees, but if I add protease inhibitor then I'll aliquot and keep at -20. remember it is not recommended to freeze-thaw protease inhibitors.....but personally I have even freeze-thawed after addition of protease inhibitors and have had no problem so far. :(




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